我试图从 SingleR 包中获取函数 CreateSinglerObject 以根据本指南工作：http ://comphealth.ucsf.edu/SingleR/SingleR_create.html
不幸的是，我收到错误“找不到”。下面是我的输入和输出。版本是 R-3.6.1。
我很感激你能给我的任何帮助。谢谢！
输入/输出
> library(Seurat)
Warning message:
In readRDS(path) : error reading from connection
> library(SingleR)
Loading required package: SummarizedExperiment
Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics
Loading required package: parallel
Attaching package: ‘BiocGenerics’
The following objects are masked from ‘package:parallel’:
clusterApply, clusterApplyLB, clusterCall, clusterEvalQ, clusterExport, clusterMap, parApply, parCapply,
parLapply, parLapplyLB, parRapply, parSapply, parSapplyLB
The following objects are masked from ‘package:stats’:
IQR, mad, sd, var, xtabs
The following objects are masked from ‘package:base’:
anyDuplicated, append, as.data.frame, basename, cbind, colnames, dirname, do.call, duplicated, eval,
evalq, Filter, Find, get, grep, grepl, intersect, is.unsorted, lapply, Map, mapply, match, mget, order,
paste, pmax, pmax.int, pmin, pmin.int, Position, rank, rbind, Reduce, rownames, sapply, setdiff, sort,
table, tapply, union, unique, unsplit, which, which.max, which.min
Loading required package: S4Vectors
Attaching package: ‘S4Vectors’
The following object is masked from ‘package:base’:
expand.grid
Loading required package: IRanges
Attaching package: ‘IRanges’
The following object is masked from ‘package:grDevices’:
windows
Loading required package: GenomeInfoDb
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material; view with 'browseVignettes()'. To cite Bioconductor, see
'citation("Biobase")', and for packages 'citation("pkgname")'.
Loading required package: DelayedArray
Loading required package: matrixStats
Attaching package: ‘matrixStats’
The following objects are masked from ‘package:Biobase’:
anyMissing, rowMedians
Loading required package: BiocParallel
Attaching package: ‘DelayedArray’
The following objects are masked from ‘package:matrixStats’:
colMaxs, colMins, colRanges, rowMaxs, rowMins, rowRanges
The following objects are masked from ‘package:base’:
aperm, apply, rowsum
Attaching package: ‘SummarizedExperiment’
The following object is masked from ‘package:Seurat’:
Assays
> singler = CreateSinglerObject(ln, annot = NULL, min.genes = 0, technology = "10X", species = "Mouse", normalize.gene.length = F, variable.genes = "de", fine.tune = F, do.signatures = F, clusters = NULL, do.main.types = T, reduce.file.size = T, numCores = SingleR.numCores)
Error in CreateSinglerObject(ln, annot = NULL, min.genes = 0, technology = "10X", :
could not find function "CreateSinglerObject"