如何分割血管python opencv

Sha*_*hah 9 python opencv image computer-vision edge-detection

我正在尝试使用Python和OpenCV对视网膜图像中的血管进行分割.这是原始图像:

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理想情况下,我希望所有的血管都像这样(不同的图像)非常明显:

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这是我到目前为止所尝试的.我拍了图像的绿色通道.

img = cv2.imread('images/HealthyEyeFundus.jpg')
b,g,r = cv2.split(img)
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然后我试图按照创建匹配滤波器这篇文章,这是输出图像是什么:

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然后我尝试进行最大熵阈值处理:

def max_entropy(data):
    # calculate CDF (cumulative density function)
    cdf = data.astype(np.float).cumsum()

    # find histogram's nonzero area
    valid_idx = np.nonzero(data)[0]
    first_bin = valid_idx[0]
    last_bin = valid_idx[-1]

    # initialize search for maximum
    max_ent, threshold = 0, 0

    for it in range(first_bin, last_bin + 1):
        # Background (dark)
        hist_range = data[:it + 1]
        hist_range = hist_range[hist_range != 0] / cdf[it]  # normalize within selected range & remove all 0 elements
        tot_ent = -np.sum(hist_range * np.log(hist_range))  # background entropy

        # Foreground/Object (bright)
        hist_range = data[it + 1:]
        # normalize within selected range & remove all 0 elements
        hist_range = hist_range[hist_range != 0] / (cdf[last_bin] - cdf[it])
        tot_ent -= np.sum(hist_range * np.log(hist_range))  # accumulate object entropy

        # find max
        if tot_ent > max_ent:
            max_ent, threshold = tot_ent, it

    return threshold


img = skimage.io.imread('image.jpg')
# obtain histogram
hist = np.histogram(img, bins=256, range=(0, 256))[0]
# get threshold
th = max_entropy.max_entropy(hist)
print th

ret,th1 = cv2.threshold(img,th,255,cv2.THRESH_BINARY)
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这是我得到的结果,显然没有显示所有的血管:

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我也试过拍摄图像的匹配滤镜版本并考虑其sobel值的大小.

img0 = cv2.imread('image.jpg',0)
sobelx = cv2.Sobel(img0,cv2.CV_64F,1,0,ksize=5)  # x
sobely = cv2.Sobel(img0,cv2.CV_64F,0,1,ksize=5)  # y
magnitude = np.sqrt(sobelx**2+sobely**2)
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这使船只更加突然出现:

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然后我尝试了Otsu阈值:

img0 = cv2.imread('image.jpg',0)
# # Otsu's thresholding
ret2,th2 = cv2.threshold(img0,0,255,cv2.THRESH_BINARY+cv2.THRESH_OTSU)

# Otsu's thresholding after Gaussian filtering
blur = cv2.GaussianBlur(img0,(9,9),5)
ret3,th3 = cv2.threshold(blur,0,255,cv2.THRESH_BINARY+cv2.THRESH_OTSU)

one = Image.fromarray(th2).show()
one = Image.fromarray(th3).show()
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大津没有给出足够的结果.它最终会在结果中包含噪音:

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如何能成功分割血管,我们对此表示赞赏.

FiR*_*iTi 2

几年前,我从事视网膜血管检测工作,有多种方法可以实现:

为了获得更好的结果,这里有一些想法: