如何在r中更改异构双字母

Question

我有一个数据框:

DF = read.table(text="S01   S02     S03    S04    S05   S06
TT     CC     TT     CT     TT     00
AC     AA     AC     CC     AA     AA
CC     TC     CC     TT     CC     00
CC     AC     CC     AC     AA     CC
GG     00     TG     TT     GG     TG
GG     GA     GG     GA     GG     GG", header=T, stringsAsFactors=F)

我想以更快的方式将所有异构值(双字母)更改为双倍"00".预期结果:

S01   S02     S03    S04    S05   S06
TT     CC     TT     00     TT     00
00     AA     00     CC     AA     AA
CC     00     CC     TT     CC     00
CC     00     CC     00     AA     CC
GG     00     00     TT     GG     00
GG     00     GG     00     GG     GG

感谢任何帮助!

Answer 1

我将假设这是基因数据.这样可以轻松构建所有异构基对,并使用正则表达式替换它们:

bases <-c("A","C","G","T")
b1 <- rep(bases, 4)
b2 <- rep(bases, each=4)
hetero<- paste0(b1[b1!=b2],b2[b2!=b1])

DF[] <- lapply(DF,gsub, pattern=paste0(hetero,collapse="|"),replacement="00")

要么

m <- as.matrix(DF)
m[m %in% hetero] <- "00"
res <- as.data.frame(m)

基准

因为基准测试很有趣,并且在这个线程中有很多不同的解决方案.令人惊讶的结论:差异不是很大,获胜者是DavidH(第二名康拉德).

具有1000列和1000行的数据框上的结果:

Unit: milliseconds
    expr      min       lq     mean   median       uq      max neval   cld
 MrFlick 402.0281 477.4867 494.6892 484.5600 504.6442 592.0486    50    d 
  Heroka 227.1143 298.8655 333.7875 309.4572 375.5734 459.6164    50   c  
 Heroka2 696.2465 710.0094 733.5981 717.8195 775.4891 803.7156    50     e
  DavidH 124.7802 127.9947 137.0511 130.3487 134.9696 210.5570    50 a    
  Konrad 144.0454 214.8844 231.9005 221.9659 291.3668 344.4238    50  b   
 Konrad2 699.5301 711.7724 750.1756 736.2112 787.4504 849.0606    50     e


#Data generated:

b1 <- rep(bases, 4)
b2 <- rep(bases, each=4)
all <- paste0(b1,b2)
largedat <- data.frame(matrix(sample(all,1000000,T),ncol=1000))

#benchmarking code

tests <- microbenchmark(
  MrFlick = MrFlick(largedat),
  Heroka = Heroka (largedat),
  Heroka2= Heroka2(largedat),
  DavidH=DavidH(largedat),
  Konrad = Konrad(largedat),
  Konrad2 = Konrad2(largedat),
  times=50)
#  Functions used:

MrFlick <- function(DF){
  as.data.frame(gsub("^(.)(?!\\1).$","00", as.matrix(DF), perl=T))
}

Heroka <- function(DF){
  bases <-c("A","C","G","T")
  b1 <- rep(bases, 4)
  b2 <- rep(bases, each=4)
  hetero<- paste0(b1[b1!=b2],b2[b2!=b1])
  m <- as.matrix(DF)
  m[m %in% hetero] <- "00"
  res <- as.data.frame(m)
  res
}

Heroka2 <- function(DF){
  DF[] <- lapply(DF,gsub, pattern=paste0(hetero,collapse="|"),replacement="00")
  DF
}

DavidH <- function(DF){
  ex <- expand.grid(c("A","T","C","G"),c("A","T","C","G"))
  ex <- ex[ex[1]!=ex[2],]
  het.combs <- apply(ex,1,function(i) {paste0(i[1],i[2])} )
  map <- setNames( rep("00",length(het.combs)) , het.combs )
  fac.df<- lapply(DF, as.factor)

  fac.df <- lapply(fac.df, function(i){levels(i)[levels(i) %in% names(map)] <- map[levels(i)[levels(i) %in% names(map)]];i } )
  DF <- as.data.frame(fac.df)
}

Konrad <- function(DF){
  bases = c('A', 'C', 'G', 'T')
  homozygous = apply(cbind(bases, bases), 1, paste, collapse = '')

  DF = as.matrix(DF)
  DF[! DF %in% homozygous] = '00'
  DF
}

Konrad2 <-function(DF){
  bases = c('A', 'C', 'G', 'T')
  homozygous = apply(cbind(bases, bases), 1, paste, collapse = '')
  DF = data.frame(lapply(DF, function (x) ifelse(x %in% homozygous, x, '00')))
}